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The extracts of the distinct samples were centrifuged at 7000 rpm for 25 min at 4 C

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Moreover, the AP, which is situated in the caudal brainstem and is part of the dorsal vagal complex (DVC), which also includes the nucleus of the solitary tract (NTS) and the dorsal motor nucleus of the vagus (DMV), acts as a critical nucleus where peripheral ghrelin transmits orexigenic messages